Genetic Variability, Evolution, and Biological Effects of Grapevine fanleaf virus Satellite RNAs
نویسندگان
چکیده
منابع مشابه
Characterization of the Full Length Coat Protein Gene of Iranian Grapevine fanleaf virus isolates, genetic variation and phylogenetic analysis
The full-length coat protein gene of Grapevine fanleaf virus (GFLV) isolates from Iran was characterized byreverse transcription polymerase chain reaction (RTPCR) and sequencing. The expected 1515 bp coatprotein (CP) gene amplicon was obtained for 16 isolates out of 89 that were identified by double antibodysandwich enzyme-linked immunesorbent assay (DASELISA) in a population ...
متن کاملImmunodetection of grapevine fanleaf virus satellite RNA-encoded protein in infected Chenopodium quinoa.
An antiserum was raised against a fusion protein containing the C-terminal half of the protein (P3) encoded by the satellite RNA of grapevine fanleaf virus (GFLV; F13 isolate) and the N-terminal portion of the CI repressor of phage lambda. This antiserum specifically recognized P3 synthesized in the in vitro wheatgerm translation system and also in infected Chenopodium quinoa plants. In these p...
متن کاملReplication of grapevine fanleaf virus satellite RNA transcripts in Chenopodium quinoa protoplasts.
A set of full-length cDNA clones of the satellite RNA of grapevine fanleaf nepovirus isolate F13 (GFLV-F13) was constructed with a variable number of additional, non-viral nucleotides at the 5' and 3' ends. The biological activity of the RNAs transcribed from these constructs was tested in Chenopodium quinoa protoplasts using a helper virus. When inoculated with arabis mosaic virus S (ArMV-S) R...
متن کاملThe Occurrence of Grapevine Fanleaf Virus in Washington State Vineyards
Grapevine degeneration in grapevines caused by Grapevine fanleaf virus (GFLV) has been documented in many viticultural regions worldwide (Andret-Link et al. 2004). It is one of the major economically important virus diseases affecting the longevity of grapevines and reducing the fruit yield and fruit quality. Infected grapevines show a range of foliar symptoms consisting of leaf deformation, ye...
متن کاملQuasispecies nature of the genome of Grapevine fanleaf virus.
Genetic diversity was characterized in 14 isolates of Grapevine fanleaf virus (GFLV) recovered from grapevine (Vitis vinifera). Virions were collected by immunocapture, and a 1557 bp fragment containing part of the viral coat protein gene and part of the untranslated region to its 3' side was amplified by RT-PCR. Sequence variation among isolates was characterized by restriction fragment length...
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ژورنال
عنوان ژورنال: Phytopathology®
سال: 2013
ISSN: 0031-949X,1943-7684
DOI: 10.1094/phyto-11-12-0310-r